Mukhtabar : Journal of Medical Laboratory Technology

Effectiveness of Lime Concentration As A Modification of Turk's Reagent in Leucocyte Type Count Examination

2024-09-12T14:42:31+07:00

Background & Objective: Turk solution has a composition of glacial acetic acid, gentian violet, and distilled water. Lime (Citrus aurantifolia S) has a pH of 2.0 which is almost the same as acetic acid. Both components are weak acids that can lyse blood cells other than white blood cells. This study aims to determine the effectiveness of lime concentration as a modification of Turk's reagent in counting the number of leukocytes and knowing the concentration of 1%, 2%, or 3% which is more effective in counting the number of leukocytes.

Method: This study aims to provide an overview of the effectiveness of lime concentration as a modification of turk reagent composition in counting the number of leukocytes. The research method used is the Counting Room Method. This research was conducted in August 2024 at the clinical pathology laboratory of the Muhammadiyah Makassar Polytechnic. The number of samples in this study was 4 blood samples obtained from 4 correspondents with treatment, lime concentrations namely 1%, 2%, and 3%, and turk reagent as control.

Result: At 1% concentration, the average difference in leucocyte count between the control and the sample was 1,150 cells/mm³, at 2% concentration a difference of 2,788 cells/mm³ was obtained with the control, and at 3% concentration a difference of 3,638 cells/mm³ was obtained.

Conclusion: It can be concluded that the concentration of lime that is effective as a modification of Turk's reagent in counting the number of leukocytes is a concentration of 1%.

The Effect of Stress Levels on Blood Glucose Levels in Medical Laboratory Technology Final-Year Students

2024-09-06T15:20:58+07:00

Background & Objective: Final-year students are a group that experiences stress due to academic life, responsibilities, and demands from the academic world, such as completing theses, fieldwork, and other academic burden. In addition, the current pandemic may cause stress related to communication and daily life changes. Stress increases the release of epinephrine, catecholamine, glucagon, glucocorticoids, beta-endorphin, and growth hormone, which causes excessive cortisol production, increasing blood sugar levels. This study aimed to determine the relationship between stress levels and blood sugar levels in undergraduate students in medical laboratory technology.

Method: This research method used a cross-sectional approach, with 35 samples that fit the inclusion criteria. The stress measuring tool used was the Depression Anxiety Stress Scale (DASS) questionnaire. Blood glucose examination using the Glucose Oxidase Peroxidase Aminoantypirin method. The analysis used in this study is the correlation test using the Chi-square test

Result: Stress levels in students show normal stress at as much as 11% mild stress at 26% moderate stress at 46%, and severe stress at as much as 17%. The results of bivariate analysis between stress levels and blood glucose levels were p=0.012 (p<0.05).

Conclusion: there is a significant relationship between stress levels and blood glucose levels in final-year students of D4 Medical Laboratory Technology at Jenderal Achmad Yani University, Cimahi. The results of this study became one of the prediabetes screening data. The need for stress management in students can be one of the factors to avoid the risk of diabetes.

Effect of Variation Natrium Sitrat Concentration and Centrifugation Time on Prothrombin Time (PT) Value

2024-09-11T14:35:18+07:00

Background & Objective: Prothrombin Time (PT) test is one of the hemostasis tests used to determine whether there is a deficiency of extrinsic clotting factors and co-pathway clotting factors, namely factors I, II, V, VII, and X. The pre-analytical stage that must be considered in the hemostasis examination is the concentration of anticoagulant used and the centrifugation process. This study was conducted to determine the effect of variations in sodium citrate anticoagulant concentration and centrifugation time on Prothrombin Time (PT) values.

Method: The anticoagulants used in this study were 3.2% and 3.8% sodium citrate. Centrifugation is the process of separating cells from plasma. The effect of improper centrifugation is to increase the number of platelets, so that platelet factors contained in platelets will accelerate the formation of fibrin. The research conducted is a pseudo-experimental study by giving treatment to blood specimens that will be added with anticoagulant Sodium Citrate concentrations of 3.2% and 3.8% with a blood ratio of 9:1, then centrifuged at 3000 rpm and given a variation of time for 15 minutes, 10 minutes, and 5 minutes. The results were tested using Saphiro Wilk and Friedman Test.

Result : Based on the research that has been done, the Asymp. Sig. value obtained in the Friedman Test is >0.05. This indicates that the treatment that has been given to the sample has no effect on the value of Prothrombin Time.

Conclusion: It can be concluded that there is no significant difference between the variation of sodium citrate concentrations of 3.2% and 3.8%, as well as in the variation of 3000 rpm centrifugation time of 15 minutes, 10 minutes, and 5 minutes on the value of Prothrombin Time (PT).

Comparison of Carbol Fuchsin Concentration on the Results of Acid-Fast Bacteria Staining (AFB)

2024-09-11T14:32:55+07:00

Background & Objective: Infections caused by the bacterium Mycobacterium tuberculosis are known as tuberculosis. (TB). The treatment process takes a long time because this bacillus bacteria is very strong. Compared to other parts of the human body, this bacteria more frequently attacks the lungs. Patients who test positive for acid-fast bacilli (AFB) are the cause of pulmonary tuberculosis (TB). The aim of this research is to determine how the concentration of Carbol Fuchsin differs in the results of Acid-Fast staining. (BTA).

Method: This research was conducted in a laboratory and used purposive sampling techniques with the Ziehl-Neelsen staining method, resulting in 5 positive acid-fast bacilli (AFB) samples.

Result: The results of this study indicate that the best concentration is 1.5% carbol fuchsin, so it can be concluded that the optimal staining concentration is 1.5% carbol fuchsin.

Conclusion: Based on the research conducted, it was concluded that the higher the concentration of carbol fuchsin, the stronger it binds to the cell walls that have a lipid layer, thus being able to bind the red colour.

Identification of Malassezia sp. as a Cause of Dandruff Among Students at Muhammadiyah Polytechnic Makassar

2024-10-08T07:37:50+07:00

Background & Objective: Dandruff is a scalp condition attributed to the fungus Malassezia sp. It manifests as fine to coarse scales that are grayish or white, resulting from the shedding of the stratum corneum layer on the scalp. Factors contributing to the onset of dandruff include the proliferation of Malassezia fungi, sebaceous gland activity, and individual predisposition. This study aimed to identify Malassezia sp. responsible for dandruff among female students at the Muhammadiyah Polytechnic of Makassar.

Method: The research employed a laboratory observational design utilizing a purposive sampling technique. Dandruff samples were collected from 10 female students and cultured on SDA (Sabouraud Dextrose Agar) media, followed by microscopic examination of the resulting colonies.

Result: The findings revealed that none of the 10 dandruff samples contained Malassezia sp.; instead, contamination by Aspergillus fumigatus was observed.

Conclusion: Therefore, it can be concluded that Malassezia sp. was not identified as a causative agent of dandruff in female students at the Muhammadiyah Polytechnic of Makassar

Optimization of Concentration and Staining Duration of Methyl Green in The Examination of Escherichia coli DNA Bands Using Agarose Gel Electrophoresis

2024-10-09T18:43:32+07:00

Background & Objective: Nowadays, many alternative dyes are used for staining DNA during electrophoresis, one of which is Methyl Green. Methyl Green has several advantages, including being cheaper than EtBr, having low toxicity, being non-carcinogenic, and as a cationic dye, Methyl Green is resistant to photobleaching. This study aims to determine the optimal concentration and staining duration of Methyl Green as a DNA dye for examining Escherichia coli DNA bands using agarose gel electrophoresis.

Method: The research method used is experimental. Amplified Escherichia coli 16S rRNA gene DNA, sized 584 bp, which has undergone electrophoresis, was stained with Methyl Green dye at concentrations of 0.10%, 0.15%, 0.20%, 0.25%, 0.00015%, 0.00020%, 0.00025%, and 0.00030% with varying immersion times of 10, 15, 20, and 25 minutes.

Result: The resulting DNA bands were analyzed or measured for surface area using ImageJ software. The mean value for each experimental group was calculated. The highest mean value was used as the basis for determining the most optimal condition.

Conclusion: This study concludes that the optimal concentration and staining duration of Methyl Green, based on the highest mean value of 19,844,845, is 0.00030% Methyl Green with a staining duration of 25 minutes.