Comparison of Antioxidant Power Extract of Ethanol Extract, Hard Shell and Seeds on Melinjo ( Gnetum gnemon L.) with DPPH Method (1,1-diphenyl-2-picrylhydrazyl)

Windy Widyawatin; Marlina Indriastuti

doi:10.52221/dwj.v1i1.225

Authors

Windy Widyawatin STIKes Muhammadiyah Ciamis
Marlina Indriastuti STIKes Muhammadiyah Ciamis

DOI:

https://doi.org/10.52221/dwj.v1i1.225

Keywords:

antioxidants, melinjo, DPPH

Abstract

The human body cannot be avoided from exposure to free radicals in everyday life. Free radicals are very dangerous for the body, one of which causes degenerative diseases. One of the plants that is thought to be able to inhibit free radicals is the melinjo plant. The processing of melinjo plants in the community is only used as a vegetable, so there is a need for research on the benefits of antioxidants in melinjo plants. The purpose of this study was to explore the parts of melinjo which had the best antioxidant activity found in the soft shell, hard shell and seeds of melinjo (Gnetum gnemon L.) with different solvent concentrations. The research method used was to prepare test samples by maceration process using 50% and 70% ethanol, then the extracts were tested for antioxidants using the DPPH method. Data analysis was performed using the ANOVA statistical test with a 98% confidence level. The results showed that the optimal percentage of free radical inhibition was obtained in 50% ethanol solvent, the outer shell of melinjo was 53.29%, and the IC₅₀was 318.18 ppm. Based on the One way Anova test (p>0.02) there was no difference between the use of 50% and 70% ethanol solvent on the antioxidant activity of the outer shell, hard skin and melinjo seed samples.

Author Biographies

Windy Widyawatin, STIKes Muhammadiyah Ciamis

Marlina Indriastuti, STIKes Muhammadiyah Ciamis

References

Andasari, S. D., Hermanto, A. A., & Wahyuningsih, A. (2020). Perbandingan Hasil Skrining Fitokimia Daun Melinjo (Gnetum gnemon L.) Dengan Metode Maserasi Dan Sokhletasi. CERATA Jurnal Ilmu Farmasi, 11(2), 27–31.

Dewi, C., Utami, R., & Riyadi, N. H. (2012). Aktivitas Antioksidan dan Antimikroba Ekstrak Melinjo (Gnetum gnemon L.). Jurnal Teknologi Hasil Pertanian, 5(2), hal 74-81. https://jurnal.uns.ac.id/ilmupangan/article/view/13554/11298

Fitria, V., Ismail, R., & Nugraha, D. (2017). Uji Aktivitas Mukolitik Infusa Daun Karuk (Piper Sarmentosumroxb. Ex. Hunter) Pada Mukus Usus Sapi Secara In VitrO. DII Farmasi Stikes Muhammadiyah: Ciamis, 9-11.

Kertawati, Lestari, A. A., Herlina, Amriani, A., & Wijaya, D. P. (2022). Accute toxicity of extract from melinjo (Gnetum gnemon L) leaf with fixed dose procedure method. Indonesian Journal of Pharmaceutical Science and Technology, 9(3), 140–148. http://jurnal.unpad.ac.id/ijpst/

Kunarto, B., Sutardi, S., Supriyanto, S., & Anwar, C. (2019). Optimasi Ekstraksi Berbantu Gelombang Ultrasonik pada Biji Melinjo Kerikil (Gnetum gnemon L., ‘Kerikil’) Menggunakan Response Surface Methodology. Jurnal Aplikasi Teknologi Pangan, 8(3), 104. https://doi.org/10.17728/jatp.5122

Kunnaryo, H. J. B., & Wikandari, P. R. (2021). Antosianin dalam Produksi Fermentasi dan Perannya sebagai Antioksidan. Unesa Journal of Chemistry, 10(1), 24–36. https://doi.org/10.26740/ujc.v10n1.p24-36

Listiana, L., Wahlanto, P., Ramadhani, S. S., & Ismail, R. (2022). Penetapan Kadar Tanin Dalam Daun Mangkokan (Nothopanax scutellarium Merr) Perasan Dan Rebusan Dengan Spektrofotometer UV-Vis. Pharmacy Genius, 1(1), 62–73

Noviati., Qian, H., & Nihorimbere, V. (2004). Antioxidant power of phytochemicals from Psidium guajava leaf. Journal of Zhejiang University: Science, 5(6), 676–683. https://doi.org/10.1631/jzus.2004.0676

Permatasari, A., Batubara, I., Nursid, M., Kimia, D., Matematika dan Ilmu Pengetahuan Alam, F., Pertanian Bogor, I., Studi Biofarmaka Tropika Institut Pertanian Bogor, P., Besar Riset Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan, B., & Kelautan dan Perikanan, K. (2020). Pengaruh Konsentrasi Etanol dan Waktu Maserasi terhadap Rendemen, Kadar Total Fenol dan Aktivitas Antioksidan Ekstrak Rumput Laut Padina australis. A Scientific Journal, 37(2), 78–84. https://doi.org/10.20884/1.mib.2020.37.2.1192

Riwanti, P., Izazih, F., & Amaliyah. (2020). Pengaruh Perbedaan Konsentrasi Etanol pada Kadar Flavonoid Total Ekstrak Etanol 50,70 dan 96%. Journal of Pharmaceutical Care Anwar Medika, 2(2), 82–95.

Safiera, A. A. (2016). Aktivitas antioksidan dan kemampuan proteksi terhadap kerusakan DNA dari protein isolat biji melinjo (Gnetum gnemon L.) terhidrolisis menggunakan alkalase terimobilisasi. 5(2), 68–74.

Sani, E. Y., & Kunarto, B. (2018). Ekstraksi Antosianin Kulit Melinjo Merah Dan Stabilitas Warnanya Pada Berbagai Lama Pemanasan. Jurnal Pengembangan Rekayasa Dan Teknologi, 13(2), 33. https://doi.org/10.26623/jprt.v13i2.928

Yuliani, W., & Ismail, R. (2023). Uji Aktivitas Antijamur Fungi Endofit Tanaman Sarang Semut (Myrmecodia pendans) Terhadap Jamur Candida albicans. Pharmacy Genius, 2(1), 31–42.

Yusuf, A. L., Nugraha, D., Wahlanto, P., Indriastuti, M., Ismail, R., & Himah, F. A. . (2022). Formulasi Dan Evaluasi Sediaan Gel Ekstrak Buah Pare (Momordica Charantia L.) Dengan Variasi Konsentrasi Carbopol 940. Pharmacy Genius, 1(1), 50–61.