Differences in Urine Protein Levels of Hemodialysis Patients With Acetic Acid and Lemon Juice Examination


  • Nurani Ai Erlinawati STIKes Karsa Husada Garut
  • Mamay Mamay STIKes Karsa Husada Garut
  • Nita Wahyuni STIKes Karsa Husada Garut




Acetic acid 6%, Hemodialysis, Lemon, Urine protein


Background and Objectives: Urine protein examination is a routine examination performed to determine kidney function. The principle of urine protein examination is that the protein in urine is denatured by heating and adding acid. Lemon fruit has a high acid content and lemon has similar properties to acetic acid, namely properties as a weak acid with a pH of 3.0. The direction of this research is to understand the comparison of urine protein using lemon juice and acetic acid solution reagents.

Methods: This research uses experimental techniques because urine is treated directly by testing using a 6% acetic acid solution (control) and lemon juice solution.

Results: the examination results using lemon juice solution are the same as the examination results using 6% acetic acid solution (control), using the Wilcoxon test, the Asymp.sig (2-tailed) value is 1.000.  With the provisions of the test >0.05, Ho is accepted and Ha is rejected, so testing the difference in urine protein levels of hemodialysis patients with acetic acid and lemon juice examination can be carried out.

Conclusion: The results of urine protein examination with acetic acid (control) and lemon juice as an alternative reagent. There is no difference in the results of urine protein examination using 6% acetic acid reagent and lemon juice.

Author Biographies

Nurani Ai Erlinawati, STIKes Karsa Husada Garut

Program Studi D3 Analis Kesehatan

Mamay Mamay, STIKes Karsa Husada Garut

Prodi D3 Analis Kesehatan


Aziz, A., & Kamran, M. (2019). Association of high levels of spot urine protein with high blood pressure, mean arterial pressure, and pulse pressure with the development of diabetic chronic kidney …. Current Diabetes Reviews. https://www.ingentaconnect.com/content/ben/cdr/2019/00000015/00000006/art00007

Gold, N. B., Blumenthal, J. A., Wessel, A. E., Stein, D. R., & ... (2018). Acute pancreatitis in a patient with maple syrup urine disease: a management paradox. The Journal of …. https://www.sciencedirect.com/science/article/pii/S0022347618303184

He, T., Pejchinovski, M., Mullen, W., Beige, J., & ... (2021). Peptides in plasma, urine, and dialysate: toward unraveling renal peptide handling. PROTEOMICS …. https://doi.org/10.1002/prca.202000029

Hosseini, S. S., Ghaemi, E., Noroozi, A., & Niknejad, F. (2019). Zinc oxide nanoparticles inhibition of initial adhesion and ALS1 and ALS3 gene expression in Candida albicans strains from urinary tract infections. Mycopathologia. https://doi.org/10.1007/s11046-019-00327-w

Kanno, Y., & Kanda, E. (2019). Comparison of accuracy between pre-hemodialysis and post-hemodialysis levels of nutritional factors for prediction of mortality in hemodialysis patients. Clinical Nutrition. https://www.sciencedirect.com/science/article/pii/S0261561417314322

Laskowski, J., Philbrook, H. T., & ... (2019). Urine complement activation fragments are increased in patients with kidney injury after cardiac surgery. American Journal of …. https://doi.org/10.1152/ajprenal.00130.2019

Lestari, A. T. B. (2020). Pengaruh Inhalasi Aromaterapi Lemonterhadap Penurunan Dismenorea Pada Siswi Kelas X Di Sman 12 Bandar Lampung Tahun 2020. repository.poltekkes-tjk.ac.id. https://repository.poltekkes-tjk.ac.id/id/eprint/2731/

Medina, S., Heras-Gomez, I. D. las, Casas-Pina, T., & ... (2020). Urinary oxylipin signature as biomarkers to monitor the allograft function during the first six months post-renal transplantation. Free Radical Biology …. https://www.sciencedirect.com/science/article/pii/S0891584919316156

Oto, J., Fernandez-Pardo, A., Roca, M., Plana, E., & ... (2020). Urine metabolomic analysis in clear cell and papillary renal cell carcinoma: A pilot study. Journal of …. https://www.sciencedirect.com/science/article/pii/S1874391920300919

Purwati, D. D., Mustika, A., Hakim, L., & ... (2022). Correlation of serum nitric oxide and urine malondialdehyde levels in non-hemodialysis chronic kidney disease patients. Molecular and Cellular …. https://www.cellbiopharm.com/ojs/index.php/MCBS/article/view/226

Raikou, V. D., & Kyriaki, D. (2019). Factors related to peripheral arterial disease in patients undergoing hemodialysis: the potential role of monocyte chemoattractant protein-1. Hypertension Research. https://www.nature.com/articles/s41440-019-0259-x

Rodan, L. H., Aldubayan, S. H., Berry, G. T., & ... (2018). Acute illness protocol for maple syrup urine disease. Pediatric Emergency …. https://journals.lww.com/pec-online/Fulltext/2018/01000/Acute_Illness_Protocol_for_Maple_Syrup_Urine.15.aspx




How to Cite

Erlinawati, N. A., Mamay, M., & Wahyuni, N. (2023). Differences in Urine Protein Levels of Hemodialysis Patients With Acetic Acid and Lemon Juice Examination. Mukhtabar : Journal of Medical Laboratory Technology, 1(2), 72–78. https://doi.org/10.52221/mjmlt.v1i2.384