Comparison of Carbol Fuchsin Concentration on the Results of Acid-Fast Bacteria Staining (AFB)

Putri Rabiah Al Adawiah; Mujahidah Basarang; Juneke Gabriella Rantung

doi:10.52221/mjmlt.v2i2.691

Authors

Putri Rabiah Al Adawiah Muhammadiyah Polytechnic Makassar
Mujahidah Basarang Muhammadiyah Polytechnic Makassar
Juneke Gabriella Rantung Muhammadiyah Polytechnic Makassar

DOI:

https://doi.org/10.52221/mjmlt.v2i2.691

Keywords:

Tuberculosis, Carbol Fuchsin, Acid-Fast Bacilli

Abstract

Background & Objective: Infections caused by the bacterium Mycobacterium tuberculosis are known as tuberculosis. (TB). The treatment process takes a long time because this bacillus bacteria is very strong. Compared to other parts of the human body, this bacteria more frequently attacks the lungs. Patients who test positive for acid-fast bacilli (AFB) are the cause of pulmonary tuberculosis (TB). The aim of this research is to determine how the concentration of Carbol Fuchsin differs in the results of Acid-Fast staining. (BTA).

Method: This research was conducted in a laboratory and used purposive sampling techniques with the Ziehl-Neelsen staining method, resulting in 5 positive acid-fast bacilli (AFB) samples.

Result: The results of this study indicate that the best concentration is 1.5% carbol fuchsin, so it can be concluded that the optimal staining concentration is 1.5% carbol fuchsin.

Conclusion: Based on the research conducted, it was concluded that the higher the concentration of carbol fuchsin, the stronger it binds to the cell walls that have a lipid layer, thus being able to bind the red colour.

References

Ariyani., Inggriani, M. (2019). Perbedaan hasil deteksi pewarnaan bakteri tahan asam dan rapid antigen pada pasien daignosa tuberkulosis paru. Jurnal Mitra Kesehatan, 1(2), 101-105.

Kalma, K., & Adrika, A. (2019). Perbandingan Hasil Pemeriksaan Basil Tahan Asam Antara Spesimen Dahak Langsung Diperiksa Denga Ditunda 24 Jam. Jurnal Media Analis Kesehatan, 9(2), 130–135. https://doi.org/10.32382/mak.v9i2.682

Nizar, M. (2017). Pemberantasan dan Penanggulangan Tuberkulosis Edisi Revisi. Gosyen Publisihing.

Pralambang, S. D., & Setiawan, S. (2021). Faktor Risiko Kejadian Tuberkulosis di Indonesia. Jurnal Biostatistik, Kependudukan, Dan Informatika Kesehatan, 2(1), 60. https://doi.org/10.51181/bikfokes.v2i1.4660

Purba, D., & Manurung2, D. B. S. (2017). Perbandingan Pemeriksaan Basil Tahan Asam Metodedirect Smear Dan Metode Imunochromatographi Test Pada Tersangka Penderita Tuberkulosis Paru Di Upt. Kesehatan Paru Masyarakat Dinas Kesehatan Provinsi Sumatera Utara. IEEE International Conference on Acoustics, Speech, and Signal Processing (ICASSP) 2017, 41(2), 84–93.

Selvakumar, N., Rahman, F., Rajasekaran, S., Narayanan, P. R., & Frieden, T. R. (2002). Inefficiency of 0.3% carbol fuchsin in Ziehl-Neelsen staining for detecting acid-fast bacilli. Journal of Clinical Microbiology, 40(8), 3041–3043. https://doi.org/10.1128/JCM.40.8.3041-3043.2002