Effectiveness of Lime Concentration As A Modification of Turk's Reagent in Leucocyte Type Count Examination

Chaerul Arham; Nur Qadri Rasyid; Rifo Rianto; Rahma Moito

doi:10.52221/mjmlt.v2i2.695

Authors

Chaerul Arham Muhammadiyah Makassar Polytechnic
Nur Qadri Rasyid Muhammadiyah Makassar Polytechnic
Rifo Rianto Muhammadiyah Makassar Polytechnic
Rahma Moito Muhammadiyah Makassar Polytechnic

DOI:

https://doi.org/10.52221/mjmlt.v2i2.695

Keywords:

Keywords: Lime, Turk's Solution, Modified, Leukocytes

Abstract

Background & Objective: Turk solution has a composition of glacial acetic acid, gentian violet, and distilled water. Lime (Citrus aurantifolia S) has a pH of 2.0 which is almost the same as acetic acid. Both components are weak acids that can lyse blood cells other than white blood cells. This study aims to determine the effectiveness of lime concentration as a modification of Turk's reagent in counting the number of leukocytes and knowing the concentration of 1%, 2%, or 3% which is more effective in counting the number of leukocytes.

Method: This study aims to provide an overview of the effectiveness of lime concentration as a modification of turk reagent composition in counting the number of leukocytes. The research method used is the Counting Room Method. This research was conducted in August 2024 at the clinical pathology laboratory of the Muhammadiyah Makassar Polytechnic. The number of samples in this study was 4 blood samples obtained from 4 correspondents with treatment, lime concentrations namely 1%, 2%, and 3%, and turk reagent as control.

Result: At 1% concentration, the average difference in leucocyte count between the control and the sample was 1,150 cells/mm³, at 2% concentration a difference of 2,788 cells/mm³ was obtained with the control, and at 3% concentration a difference of 3,638 cells/mm³ was obtained.

Conclusion: It can be concluded that the concentration of lime that is effective as a modification of Turk's reagent in counting the number of leukocytes is a concentration of 1%.

References

Aliviameita, A. & Puspitasari, 2019. Buku Ajar HEMATOLOGI. Septi Budi Sartika, M.p ed. Jawa Timur: UMSIDA Press

Artati, Naim, H. N., Yusril, M. & Armah, Z., 2022. Waktu Simpan Whole Blood Cell (WBC) Terhadap Jumlah Leukosit, Eritrosit Dan Trombosit. Media Analis Kesehatan, Volume 13, p. 2

Kahfi, M. S., Aryani, D. & Purnomo, F. O., 2022. Variasi Konsentrasi Air Perasan Jeruk Nipis (Citrus Aurantifolia Swingle) Sebagai Pengganti Komposisi Larutan Turk Untuk Hitung Jumlah Leukosit Di Laboratorium Rs Hasanah Graha Afiah. Jurnal Kesehatan Tambusai, 3(1), pp. 117-118

Lestari, R. K., Amalia, E., Yuwono. 2018. Efektifitas Jeruk Nipis (citrus aurantifolia swingle) sebagai Zat Antiseptik Pada Cuci Tangan. JKK, Volume 5, No 2. pp:55-65.

Nugraha, G., 2017. Panduan Pemeriksaan Laboratorium Hematologi Dasar. Jakarta: a

Nurbidayah, M. & Maulida, I., 2019. Penggunaan Air Perasan Lemon (Citrus Limon) Sebagai Reagen Alternatif Pengganti Larutan Turk Untuk Hitung Jumlah Leukosit. Jurnal ERGASTERI, 6(2), p. 2

Prastiwi, S. S., & Ferdiansyah, F. (2017). REVIEW ARTIKEL: KANDUNGAN DAN AKTIVITAS FARMAKOLOGI JERUK. Farmaka, 15, 2.

Salman, Y., Nadia, N. & Wahidah, R., 2021. Perbedaan Hasil Jumlah Leukkosit dengan Modifikasi Air Perasan Jeruk Nipis (Citrus aurantifolia Swingle) dan Asam Cuka sebagai Pengganti Komposisi Larutan Turk. Jurnal Kesehatan Indonesia, Volume XII, p. 1

Trisnawati, I., Hersoelistyorini, W. & Nurhidajah, 2019. Tingkat Kekeruhan, Kadar Vitamin C Dan Aktivitas Antioksidan Infused Water lemon dengan Variasi Suhu dan Lama Perendaman. Jurnal Pangan Dan Gizi dan lain-lain.